Coding

Part:BBa_K4604005:Design

Designed by: Hannah Swientek   Group: iGEM23_Freiburg   (2023-09-30)


BluB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Change of start codon to adjust to E. coli. C-terminal his-tag for detection in western blot.


Source

Taken from Mycobacterium Smegmatis, sequence was taken from the National Library of Medicine (gene ID:61603312), synthesized by IDT.